Nitrogen Fixation: Hydrosulfite as Electron Donor with Cell-free Preparations of Azotobacter Vinelandii and Rhodospirillum Rubrum.
نویسندگان
چکیده
23 The analyses reported in Table 1 indicate that the fragment I used by us had 7 or 8, rather than 6 disulfide bonds, leading to an even lower probability for return to the native structure by random reoxidation. 24 Anfinsen, C. B., E. Haber, M. Sela, and F. H. White, Jr., these PROCEEDINGS, 47, 1309 (1961). 25 Chen-Lu, T., D. Yu-Cang, and X. Geng-Jun, Sci. Sinica (Peking), 10, 332 (1961); see Wilson, S., G. H. Dixon, and A. C. Wardlow, Biochim. Biophys. Acta, 62 483 (1962). 26 Burnet, F. M., Australian J. Sci., 20, 67 (1957). 27 Lederberg, J., Science, 129, 1649 (1959). 28 Szilard, L., these PROCEEDINGS, 46, 277, 293 (1960). 29 Smithies, O., Nature, 199, 1231 (1963). 30 Schweet, R. S., and R. D. Owen, J. Cellular Comp. Physiol., 52, Suppl. 1, 199 (1957). 31 Karush, F., J. Pediatrics, 60, 103 (1962).
منابع مشابه
The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.
The requirement for both an electron donor and ATP for the enzymatic reduction of N2 to ammonia was demonstrated with extracts of Clostridium pasteurianum,1-4 Azotobacter vinelandii,5 7 and Rhodospirillum rubrum.' 8 With hydrosulfite as the electron donor and an ATP-generating system (creatine phosphokinase), N2-reducing preparations from A. vinelandii and R. rubrum were shown to catalyze a con...
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The activity of NifA, the transcriptional activator of the nitrogen fixation (nif) gene, is tightly regulated in response to ammonium and oxygen. However, the mechanisms for the regulation of NifA activity are quite different among various nitrogen-fixing bacteria. Unlike the well-studied NifL-NifA regulatory systems in Klebsiella pneumoniae and Azotobacter vinelandii, in Rhodospirillum rubrum ...
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Ammonia is generally conceded to be the "key intermediate" in biological nitrogen fixation-that is, the compound which reacts to form the first stable organic compound, glutamic acid (Wilson and Burris, 1953). However, there is virtually no information concerning the reactions functioning in the conversion of N2 to ammonia. Reproducible nitrogen fixation by a cell-free preparation from bacteria...
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The N2 fixing bacteria Klebsiella pneumoniae, Azospirillum brasilense, Rhodopseudomonas sphaeroides and Rhodospirillum rubrum, but not Azotobacter vinelandii accumulate the glutamine analogue methionine sulfoximine in the cell. In the accumulating cells methionine sulfoximine inhibits ammonium transport. Accumulation and inhibition are prevented by glutamine.
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 53 شماره
صفحات -
تاریخ انتشار 1965